产品简介: Ribonuclease A (RNase A) is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B it is not a glycoprotein. RNase A can be inhibited by alkylation of the histidine-12 or histidine-119 which are present in the active site of the enzyme. Activators of RNase A include potassium and sodium salts. The optimal temperature for activity is60°C, although the enzyme does exhibit activity from 15-70°C. The pH optimum is 7.6, with an activity range of 6-10. The highest activity is exhibited with single stranded RNA. RNase A is a very stable enzyme and can withstand temperatures up to100°C. At100°C, RNase A is most stable between pH 2.0 and 4.5 Ribonuclease A,简称 RNase A,中文名为核糖核酸酶 A。进口试剂配制,不含 DNase,用于消化 RNA,可直接使用。用于各种常见的分子生物学、细胞生物学等相关实验。 RNaseA在质粒中的终浓度推荐为100μg/ml,即在质粒溶液中按照1/100体积加入10mg/ml RNaseA即可,如50μl质粒中加入0.5μl RNaseA溶液,37℃孵育10分钟。 注意事项: 为了您的*和健康,请穿实验服并戴一次性手套操作。 *Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 我们所提供的产品仅供科研使用,不得用于临床诊断、**、食品或者化妆品等用途!